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Clinical Nursing Research
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Testing Protocols

Care of Biological Samples in a Rural Setting

Mary Beth Flanders Stepans

Wyoming State Board of Nursing, Cheyenne, Wyoming

Susan L. Wilhelm

University of Nebraska Medical Center, Scottsbluff, Nebraska

T. Kim Rodehorst

University of Nebraska Medical Center, Scottsbluff, Nebraska

Derek Smith

University of Wyoming, Laramie, Wyoming

Clarann Weinert

Montana State University, Bozeman, Montana, cweinert{at}montana.edu

The purpose of this study is to evaluate collection, transport, and storage protocols of urine and air allergen samples from infants in a rural setting. Infant urine and aeroallergen samples (n = 21) are collected in the home setting in rural areas where time and distance to the central laboratory is a consideration. Urine samples are analyzed using ELISA tests after being transported using three different techniques: (a) ambient temperature, (b) dry ice, and (c) packaged on dry ice and shipped commercially. Following initial ELISA testing for levels of cytokines, urine samples are frozen at -70 degrees Celsius for 6 months. Samples are then reanalyzed for levels of cytokines. Dust samples are analyzed for levels of aeroallergens, stored at 4 degrees for 6 months, and reanalyzed. The integrity of samples varies by biomarker, shipment temperature, and storage time, creating a high degree of variability in results.

Key Words: biomarkers • sample collection • rural • infant • respiratory

Clinical Nursing Research, Vol. 18, No. 1, 6-22 (2009)
DOI: 10.1177/1054773808327551
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